Parameter

Description

Edit Feedback Script...

Opens the Script Editor dialog. There you can create scripts for an Experiment Feedback.

Select script runtime conditions

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Free Run

Upon the experiment start the acquisition and the feedback script are started but run from here in a completely unsynchronized manner. The online image analysis or the script run itself will not slow down the actual image acquisition.

-

Synchronized

This mode will lead to strictly determined order of events depending on the chosen level of synchronization. The online image analysis and/ or the feedback script will be started after current acquisition is finished.

In contrast to the Free Run mode, a synchronized run can slow the whole acquisition down. The big advantage of the mode is, that the synchronized run ensures a predictable workflow.

Define Script Slot

Here you define the experiment feedback sequence by arranging the slots (represented by blue buttons). The blue slots run one after another the non-blue slots are run separately.

  • The Acquisition slot represents the actual image acquisition.
  • The Analysis slot represents the online image analysis.
  • The Script Run* slot represents the execution of the experiment feedback loop script. Note that the loop script will be only executed when triggered by a used observable inside the loop script.
  • The HD Writing slot represents the slot for writing the image data to your hard drive.

Allow additional loop script runs

Triggers the main loop if observables that are not part of the multidimensional experiment (e.g. frame index, time index, block index, etc.) change. Those observables could be time or temperature of the incubation. If the checkbox is not activated only experiment observables trigger the main loop script. This only applies when the acquisition is idle. This only applies when acquisition is idle.