SEM/LM system for correlative microscopy
SEM/LM system for correlative microscopy

This module enables you to locate sample positions in two different microscopes, e.g. a light microscope and a scanning electron microscope (SEM). Afterwards you can correlate the two images to one merged image. This technique is called correlative microscopy or just "CorrMic". It is used to combine the two worlds of scanning electron microscopy and light microscopy and brings it together in one image. To use the functionality, you need a license for the Connect Toolkit.

The samples can be mounted in special designed correlative holder systems (with three correlative calibration markers) from ZEISS. Also user-defined holder systems with three calibration markers can be used. Biological samples are mainly deposited on cover glasses or on TEM grids. In contrast to biological samples, the shape and size of material samples vary strongly. In respect to these requirements, the correlative holders were designed accordingly.

Example of a correlative ZEISS sample holder
Example of a correlative ZEISS sample holder