S&F Tab

Here you find helpful options and tools to draw in and relocate regions of interests (ROIs) or points of interest (POIs) within the sample image.

Options

Parameter	Description
Mirror Image	Here you can mirror the image horizontally or vertically by using the two buttons at the right. The alignment of the images depends on the microscope (upright/inverted) and orientation of the sample holder.
Keep tool	Activated: Keeps the current tool active. That is helpful if you want to draw in more than one ROI/POI.
Auto color	Activated: Uses a new color for each new element which is drawn in.
Snap to Pixel	Activated: Draws in graphical elements using the pixel grid.
Use fine calibration value	Activated: Uses the measured fine calibration. The precision of relocation and therefore the quality of the overlay image can be improved by determination of an offset value. This value describes the offset between the loaded image and the live image. The defined offset value is only valid for the loaded image which you can see in the container. If another image is loaded or if you close the dialogue, the offset value will be deleted. Determine the offset by identification of a POI (Point Of Interest) within the snapped image. To identify a POI use the buttons in the Regions section. By clicking on the Set Offset button, the stage moves to the supposed sample position. Compare the sample position within the live image with the set POI and correct the stage in that way that both shown positions are identically. Confirm the fine calibration with the OK button. Now the fine calibration is measured and the checkbox is activated. More information, see Fine Calibration of the Sample Holder.
Double click in image to move stage	Activated: Moves the stage to the position you have double clicked on.
Refocus after stage movement	Activated: Adjusts the focus automatically after the stage has moved.
Move stage in z-direction before x/y movement	Activated: Moves the stage to the load position before it moves to the next correlative calibration marker.
Show splitter view	Activated: Activates Splitter Mode in the Center Screen Area.

Regions, Find and Dimensions

Regions panel showing 'Find' tab, 'Center' and 'ROI/POI' buttons, list entries 'POI' and 'ROI', Dimension X 472.13 Y 426.90 — Regions, Find, Dimension

Regions and Find tool bar

Parameter	Description
Selection mode	Selects the ROIs or POIs in the image area. If you are currently in another mode, you can switch back to the Selection mode using this button.
Draw rectangle	Draws in a rectangle (Region of Interest (ROI)) that is always parallel to the edges of the image.
Draw marker	Draws in a marker point (Point of Interest (POI)).
Center	Moves the stage to the center of the opened image.
ROI/POI	Moves the stage to the selected ROI/POI.
Show stage position	Shows the current stage position as a rectangle in the image.

Dimension section

Here you see coordinates and dimensions of the selected graphical element in the list. If the Scaled checkbox is activated, the unit is µm, otherwise Pixel.

Parameter X: Shows the horizontal position (x coordinate) of the center of the graphical element.
Parameter Y: Shows the vertical position (v coordinate) of the center of the graphical element.
Parameter W: Shows the width of the graphical element.
Parameter H: Shows the height of the graphical element.

Graphical elements list

Here you see the list of all ROI/POI which are drawn in.