Dynamics Profiler Acquisition Wizard

Adjustment

Starts an automatic detector adjustment. When the adjustment is running, it changes to a Stop button.

Quality and Status

Displays the status of the Airyscan detector adjustment.

Detector View

Displays the current intensity distribution of the emission signal over the detector elements.

The following parameters are only available if the automatic adjustment fails:

Zoom

Sets the zoom level. Clicking the 1.x button behind the input field resets the zoom level to the default.

High Intensity Laser Range

Activated: Enables the higher laser power range between 0.2 and 100%. This setting is applied to all laser lines.

Laser

Set the required attenuation (%) for the laser.

Master Gain

Sets the master gain to control the voltage of the PMTs. Increasing the gain of the PMT corresponds to a higher voltage of the detector. The image gets brighter and you may be able to reduce the laser power.
With a higher voltage, the noise level in the image increases as the dark noise of the detector gets visible in the images, predominantly as single bright pixels. The optimum between gain and noise depends on your experimental requirements and on your sample.

Scan Speed

Sets the scan speed with the slider or input field. The corresponding values for Frame Time and Pixel Time are displayed above the slider.

Fiber Position

Displays controls for the manual adjustment of the fiber positions.

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X Position

Changes the adjustment of the fiber in x.

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Y Position

Changes the adjustment of the fiber in y.

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Store Invis Correction Position Automatically

Activated: Saves and stores the current values for the next alignment.

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Store Current Pos.

Stores the current positions of x and y for further reference.

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Move To Stored Pos.

Moves to the currently stored position and updates the X Position and Y Position accordingly.

Continuous

Starts a continuous image acquisition. When the acquisition is running, it changes to a Stop button to stop the acquisition.

Zoom

Sets the zoom level. Clicking the 1.x button behind the input field resets the zoom level to the default.

Scan Speed

Set the scan speed with the slider or input field. The corresponding values for Frame Time and Pixel Time are displayed above the slider.

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Max

Automatically sets the maximal possible scan speed. When you click this button once, it changes its color to blue. This indicates that the system always uses the highest possible scan speed as you continue to change acquisition parameters like zoom or frame size. Click the button again to deactivate this permanently active state.

Frame Size

Adjusts the frame size (in pixel) for the image acquisition. The corresponding Image Size and Pixel Size are displayed above the input fields.

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Presets

Opens a dropdown which allows you to select a frame size from a list of default frame sizes.

Sampling

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SR

Sets the sampling to 2x Nyquist for super resolution mode.

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Confocal

Sets the frame size (image resolution) to an optimal value corresponding to the optical magnification (objective), the zoom factor and the wavelengths included in the experiment. This provides an image where no spatial information is lost and no empty data is generated as optimal sampling is achieved. The confocal value is calculated for the given objective and magnification settings matching a 1 fold sampling according to 1 time Nyquist. Rectangular image dimensions are preserved.

Direction

Selects the scan direction.

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The laser scans in one direction only, then moves back with beam blanked and scans the next line.

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The laser also scans when moving backwards, i.e. the scan time is reduced by about a factor of two. Note that bi-directional scanning can result in a pixel shift between forward and backward movement (double image).

High Intensity Laser Range

Activated: Enables the higher laser power range between 0.2 and 100%. This setting is applied to all laser lines.

Laser

Set the required attenuation (%) for the laser.

Second Track

Only visible if you have defined two Airyscan tracks.
Activated: Uses also the second Airyscan track to acquire the reference image and displays the laser setting for the second track.

Master Gain

Sets the master gain to control the voltage of the PMTs. Increasing the gain of the PMT corresponds to a higher voltage of the detector. The image gets brighter and you may be able to reduce the laser power.
With a higher voltage, the noise level in the image increases as the dark noise of the detector gets visible in the images, predominantly as single bright pixels. The optimum between gain and noise depends on your experimental requirements and on your sample.

Snap Reference Image

Creates the reference image with the current settings.

Spot Measurment

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Starts the selection mode that allows you to select and move the spots in the reference image.

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+

Allows you to add new spots in the reference image. You can add a maximum of ten spots.

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Evaluate Spot

Starts a scan for the currently selected spot. When the spot scan is running, it changes to a Stop button to stop the acquisition.

Spot Table

Displays all spots and their information.

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ID

Displays the ID of the spot.

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Color

Displays the color of the spot.

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Name

Displays the name of the spot.

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X/Y

Displays the x and y coordinates of the spot.

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Quality

Displays an indication about the quality at the respective spot.

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Move Up

Moves the currently selected spot one position up in the list.

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Move Down

Moves the currently selected spot one position down in the list.

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Delete

Deletes the currently selected spot entry.

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Delete All

Deletes all spots.

High Intensity Laser Range

Activated: Enables the higher laser power range between 0.2% and 100%. This setting is applied to all laser lines. Note that selecting a value below 0.2% is not possible in this step.

Laser

Sets the required attenuation (%) for the laser.

Time Resolution

Selects which time resolution is used.

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Spot Measurement

Uses a pixel time of 1.2 μs and a maximum measurement time of 300 seconds. This option is the recommended default for spot measurement.

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ω_r Calibration

Uses a pixel time of 0.5 µs and a maximum measurement time of 100 seconds. The recommended measurement time for ω_r calibration is 60 seconds.

Measurement Time

Sets the measurement time for the experiment.

Reference Image

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Snap

Acquires a new reference image.

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Create a Reference Image after Spot Measurement

Activated: Automatically acquires a second reference image after the spot experiment is finished.

Start Experiment

Starts the time series experiment for all spots.

Keep Positions

Activated: Saves the spot positions defined in the wizard and takes them over into the current experiment in ZEN.

Keep Acquisition Settings

Activated: Saves the acquisition settings defined in the wizard and takes them over into the current experiment in ZEN.