The Image Analysis tool allows you to perform an analysis interactively. It runs the selected analysis setting with all the steps that have been marked as interactive in the setup. Steps that you have not marked as interactive in the Image Analysis Wizard are run with the values predefined in the image analysis setting. The setting does not pause to allow you to change these values interactively.

Start Interactive Analysis also allows you to directly execute an image analysis on a czi image without predefining an image analysis setting. For that you need to have an image analysis setting where all steps are marked as interactive. Then it is possible to modify every step of the Image Analysis Wizard during the interactive analysis and do a one-time image analysis on the dataset without creating a new setting. In order to retrieve an image analysis setting from an already analyzed dataset, see Creating an Image Analysis Setting from an Analyzed Image.

Note: When you analyze an image interactively, the modifications of the settings during the interactive analysis are not saved.

1. You have defined an image analysis setting where all analysis steps you want to adjust interactively are marked as interactive.

1. On the Analysis tab, open the Image Analysis tool.
2. For Setting, select your image analysis setting.
3. Click on Start Interactive Analysis.
4. The Image Analysis Wizard opens with all the steps that are defined as interactive in the setting.
4. Modify your settings for each step and click Next to get to the following step in the wizard.
5. At the end, click Finish to close the wizard.

1. You have now analyzed your image interactively and the results of this analysis are displayed.