1. After the image acquisition you can adapt the size of image and surrounding area with the zoom keys F7 and F8 to your needs.
  2. Keep a sufficient overlap area of the live image with the stored image.
  1. Click on the Start Experiment button to start the Panorama acquisition.
  2. You will see the reduced display of the start image in the Center Screen Area.
  3. The displayed image is a live image. You can still change position and focus.
  4. Click on the Acquire Tile Image button in the Center Screen Area to acquire the first tile image.
  5. The image will be acquired and stored. The live image is still active as an overlay to the stored image.
  6. Move the blue frame with the active live image in the desired direction aside the stored image by using the mouse.
  7. Now move the sample to the corresponding neighbor position using the microscope stage.
  8. Try to position the structures in the overlap region as good as possible.
  9. Click again on the Acquire Tile Image button to acquire this tile to the image.
  10. Move the blue frame to the next position.
  11. Continue these steps until you acquired the desired panorama image of your sample.
  12. After acquiring the last tile image click on the Stop button to close the live mode.
  13. Finally end the experiment via the Stop button on the Acquisition tab.
  14. As a result you now see the recorded panorama image in a new image container.
  15. Save the image as a raw image (*.czi).
  1. You have successfully acquired and stored a panorama image.