Sample Holder Calibration - Options & Marker Detection

Step 1: Options

Sample Holder Calibration Wizard Options

Option	Description
Save marker images	Activated: the marker images are saved during the calibration. The images can be used to check the calibration afterwards. Click on the Select Folder (...) button to select a storage folder.
Move the stage to load position before x/y movement	Activated: the stage will move to load position before moving to the next correlative calibration marker. In case of using an AxioObserver, the objective revolver moves to load position.
Automatic movement to next marker	Activated: By clicking on the Next button, within the wizard the stage moves automatically to the next calibration marker. Deactivated: You must use the joystick to navigate to the markers. This is necessary when using a correlative holder which has no holder data deposited.
Use Autofocus at each marker position	This option is active only if the Automatic movement to next marker position checkbox is activated. Activated: the focus is adjusted automatically after moving to the next marker position.
Use automatic marker detection	Activated: The software will detect the small calibration marker automatically.
Use settings for marker detection	This option is active only if the Use automatic marker detection checkbox is activated. Activated: shows settings for marker detection (see description below). Here you select the properties of the calibration markers.

Settings for marker detection

Only visible if the Use settings for marker detection checkbox is activated.

Option	Description
Threshold marker detection: high – low	A low threshold for marker detection is used when the dimensions of the correlative L markers cannot be recognized precisely, e.g. when the sample holder is slightly filthy.
Marker color	Here you select the color of the markers displayed in the live image. White: the marker is displayed white on a dark background. Black: the marker is displayed dark on light background. Auto: the marker color is set automatically.
Marker orientation	Here you need to set the orientation of the L-markers on your sample holder. Click on the corresponding button to select the orientation of the calibration marker which you can see in the live image

Option

Description

Threshold marker detection: high – low

A low threshold for marker detection is used when the dimensions of the correlative L markers cannot be recognized precisely, e.g. when the sample holder is slightly filthy.

Marker color

Here you select the color of the markers displayed in the live image.

White: the marker is displayed white on a dark background.

Black: the marker is displayed dark on light background.

Auto: the marker color is set automatically.

Marker orientation

Here you need to set the orientation of the L-markers on your sample holder. Click on the corresponding button to select the orientation of the calibration marker which you can see in the live image

If you click on the Next button, you will move to the next step of the wizard.