In some cases it can be helpful to not only display the well number together with the acquired images (Path: Graphics > Frequent Annotations > Carrier Container Name) but also to create certain additional annotations for different tile regions or positions, e.g. "control condition" or "experimental condition 1".
For that purpose, the software allows you to add and edit names and categories to the different tile regions/positions that have been generated.

  1. You have licensed this functionality and activated it under Tools > Toolkit Manager.
  1. In order to assign individual names to different individual positions and/or tile regions in a well plate experiment, in the Tiles tool click on the respective name in the Tile Regions or Positions list. You can now edit the name field and press Enter to finish.
  2. Repeat this step to rename different tile regions or positions.
  3. To assign or edit categories of your tile regions/ positions, first select all desired tile regions/ positions that should be grouped in the same category.
  4. Under the Tile Regions/Positions list, in the Properties section Category, click on the Options button and select New from the dropdown list.
  5. The New Category window opens.
  6. Enter a Name and add a Description for the selected tile regions/ positions.
  7. Assign a Color for the new category by clicking on the color bar and choosing a preferred color.
  8. Click on OK to create the new category.
  9. The New Category window closes and the new category is created.
  10. As Category choose the desired category for the selected tile regions/ positions from the dropdown list.
  1. The chosen category is now assigned to the selected tile regions/ positions.

  1. To display the name of your Tile Region/ Position later in your acquired image(s), go to Graphics > Frequent Annotations > More… and select Image.Scene.Name from the metadata list.
  2. Note that a predefined category can also be applied to a differentiated selection of Tile Regions/ Positions from more than one well.
    Note also, that the assigned color is only used as a feature in the Tiles tab (Left Tool Bar Area).
  3. To display a Tile Region/ Position Category feature (Name and/or Description) in your acquired image, you go to Graphics > Frequent Annotations > More…. Type “category” in the search bar and select the desired feature to be displayed. (Although the option "Color" is given, no reasonable element will be displayed by the software.)
  4. To adjust parameters of your annotations (e.g. font size), right-click on it and go to Format > Graphical Elements.

Displaying categories in the Tiles/ Positions List (Left Tool Area)

  1. You have selected several different positions or tile regions and assigned different categories.
  1. Under Positions or Tile Regions of the Tiles tool, select a position or tile region.
  2. Right-click on the selected position/ tile, choose Sort and select By Category
  1. The positions/ tiles will be sorted alphabetically according to the assigned categories.