Local and Global Focus Surfaces
Introduction
Local Focus Surface
To acquire large tile regions on tilted or uneven specimens, you need to assign individual z-values to each tile of a tile region efficiently. ZEN will help you do this by creation of a Local Focus Surface. Note that a local focus surface is always associated with precisely one tile region. Thus, you need to create a focus area separately for each tile region.
Global Focus Surface
To create a focus area covering the entire sample, you need to create a Global Focus Surface. Global focus surfaces are based on a sample carrier template (e.g. for slides or multiwell plates) and result in a focus surface that is valid for the entire sample carrier and therefore for all the tile regions and positions it contains. This allows you to compensate for any tilting and curvature of the sample carrier.