Successful deconvolution depends mainly on good image quality, knowledge about the optical parameters of the sample and detailed knowledge about the type of instrument used for image acquisition. While information about the used instrument type can be easily extracted from the image metadata, optical parameters of the sample might not be known and the image quality can vary widely. Many parameters are available for deconvolution which allow you to make corrections to the image quality and adjust the algorithms to match the various optical conditions such as coverslip type or the medium, in which the sample is embedded. This wide range of parameters can be overwhelming.

With the Deconvolution (defaults) method, good initial results are achieved by using a carefully preselected set of default parameters. The parameters are automatically adapted to the following instrument types: widefield, confocal, lightsheet and ApoTome.
While these parameters usually give nice results, there are cases where further parameter changes are necessary, e.g., activating and using spherical aberration correction. In such cases, you should use the Deconvolution (adjustable) method, see Performing Configurable Deconvolution.

1. You are on the Processing tab.
2. You have acquired or opened a fluorescence image on which you wish to perform deconvolution.
3. All tools are in Show All mode.

1. In the Method tool, in the Deconvolution group, select Deconvolution (defaults).
2. Open the Parameters tool.
3. Four different algorithms for deconvolution are displayed (Nearest Neighbor, Regularized Inverse Filter, Fast Iterative, Constrained Iterative), which you can apply to your image. Note that the Constrained Iterative algorithm is only available if you have the license for the Deconvolution module.
3. Select an algorithm.
4. Open the Input tool.
5. If Set Input Automatically is activated, the currently active image is loaded as input image automatically. If the checkbox is deactivated, the container for the input image is empty. In this case continue with the next.
5. Click on the empty image container.
6. A list opens with preview images of all currently open images.
6. Click on the image to which you want to apply deconvolution.
   
7. The image is displayed in the image container and is as the input image for processing.
7. Click Apply on the top of the Processing tab.

1. Deconvolution is performed. A new image file is generated and opened automatically in the center screen area after processing. If you are satisfied with the result, save the processed image. Repeat deconvolution using the other default values to obtain different results. If you have expert knowledge, you can configure all the deconvolution settings yourself using the Deconvolution (adjustable) method.