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ZEISS Microscopy Knowledge Base

Movie Export

Using this function you can export multidimensional images (e.g. Time Series or Z-Stack images) into various file types in the form of film sequences so that you can continue to use them in other programs.

If you want to export MOV files (H264 or MPEG4 codec) successfully, download the latest release version of application FFmpeg, Windows 64-bit, Static (e.g. on https://www.ffmpeg.org/download.html). Copy ffmpeg.exe into the same folder where ZEN.exe is located (for example C:\Program Files\Carl Zeiss\ZEN 2\ZEN 2 (blue edition)).

File Type Section

AVI (MS-Video1) mode is available for 32-bit Windows operating systems only.

Parameter

Description

Format

Select the desired mode here. The following formats are available for the movie export.

  • AVI (M-JPEG compression)
  • AVI (uncompressed)
  • AVI (DV)
  • WMF (WindowsMedia)
  • MOV (H.264)
  • MOV (MPEG4)
  • AVI (MS-Video1)

Size/Rate

Select the desired format here.

-

Original Size

Not available for the file types AVI (DV) and AVI (MS-Video1).

Uses the height and width of the input image and sets the frame rate to 5 frames per second.

-

User-Defined

Not available for the file types AVI (DV) and AVI (MS-Video1).

Enter the values in the Width, Height, and Frame Rate input fields.

-

720x576/25fps (PAL 576p/25)

Uses the PAL (Phase Alternating Line) video resolution with 25 frames per second.

-

720x480/29.97fps (NTSC)

Uses the NTSC (National Television Systems Committee) video resolution with 29.97 frames per second.

-

1280x720/50fps (HD 720p/50)

Not available for the file types AVI (DV) and AVI (MS-Video1).

Uses the HD (High Definition 720) video resolution with 25 frames per second.

-

1920x1080/25fps (HD 1080p/25)

Not available for the file types AVI (DV) and AVI (MS-Video1).

Uses the HD (High Definition 1080) video resolution with 25 frames per second.

-

1920x1080/29.97fps (HD 1080p/29.97)

Not available for the file types AVI (DV) and AVI (MS-Video1).

Uses the HD (High Definition 1080) video resolution with 29.97 frames per second.

Width

Only active if you have selected User-Defined in the Format dropdown list.

Here you can enter the width of the image in pixels (px).

Height

Only active if you have selected User-Defined in the Format dropdown list.

Here you can enter the height of the image in pixels (px).

Frame Rate

Only active if you have selected User Defined in the Format dropdown list.

Here you can enter the frame rate in frames per second (fps).

Quality

Only visible if you have selected AVI (M-JPEG compression) or MOV in the Mode dropdown list.

Here you can set the image quality using the slider or spin box/input field. This influences the size of the file. Although low values result in very small files, image quality may be considerably reduced.

Image Data Section

The following functions are only visible if the Show All mode is activated:

Parameter

Description

Apply Display Settings and Channel Color

Burn-in Annotations

Activated: Burns the graphic elements into the image. The pixels under the graphic element (e.g. scale bars) are overwritten. The burnt-in graphic elements cannot be subsequently modified.

Zoom

Only available if Burn-in Annotations is activated.

Sets the zoom of graphic elements for export.
The default is 100%, which means that the graphical elements are rendered as they would if the viewer zoom was 100%. When the zoom increases, the graphical elements are rendered larger (e.g. with zoom 200, graphical elements are rendered twice as large, but it corresponds to the size they would have if the viewer zoom was 50%).

Get Zoom from Viewer
Gray camera icon with circular lens and small flash rectangle

Only available if Burn-in Annotations is activated.

Gets the current zoom factor of the graphical elements in the 2D view.

Burn-in Analysis Results

Only available if Burn-in Annotations is activated.
Activated: If the image contains analysis results, the masks of the results are burned into the image.

Merged Channels Image

Only visible for multi-channel images.

Activated: Exports the pseudo color image of all selected channels.

Individual Channels Image

Only visible for multi-channel images.

Activated: Exports the individual colored image of all selected channels.

Use channel names

Only visible for multi-channel images.

Activated: Integrates the channel name in the name of the exported image.

Use Full Set of Dimensions

Activated: Includes all dimensions of the original file.

Define Subset

Activated: Creates a subset of the data, depends on the dimensions available in the image, e.g. Channels, Region, Tiles.

At least one of the three checkboxes must be activated. If the Merged Channels Image and Individual Channel Image checkboxes are activated, you can export the individual colored images and the pseudo color images in a single step.

Fitting Section

The following functions are only visible if the Show All mode is activated:

Parameter

Description

Fitting

Select the desired type of fitting here.

-

Fit All (Uniform)

Fits the image to the selected resolution. The original aspect ratio is retained.

-

Fit and Crop (Uniform to Fill)

Fits the image to the selected resolution and clips it. The original aspect ratio is not retained.

-

Fit and Stretch (Fill)

Stretches the image to the selected resolution. The original aspect ratio is not retained.

-

Crop (None)

Crops the image to the selected resolution. The original aspect ratio is retained.

Sequence Section

The following functions are only visible if the Show All mode is activated:

Change the sequence of the dimensions in which you want the movie to be created.

White upward-pointing chevron icon on dark square background button

Shifts the selected dimension up a line.

White downward-pointing chevron icon on dark square background button

Shifts the selected dimension down a line.

Mapping Section

Parameter

Description

Mapping

Select how you want the images to be assigned.

-

Fixed Duration

Enter the time per image in seconds using the spin box/input field. The total length is displayed in the Final Movie Length display field.

-

1 Frame per Image

Assigns one frame per image.

Image count

Indicates the number of images in the input image.

Final Movie Length

Indicates the total length of the resulting movie, depending on the selected image sequence and the time.

Dimensions Section

Parameter

Description

Use Full Set of Dimensions

Select this option if you want to export all dimensions without changing them.

Define Subset

Select this option if you only want to export individual dimensions or subsets of individual dimensions.

Each of the sections described below is only visible if the corresponding dimension is present in the input image.

Parameter

Description

Channels

Here you can select which channels of the input image you want to be used. All channels are selected by default. To deselect a channel, click on the relevant channel button.

Z-Position, Time, Block, Scene

Here you can select which parts of the input image you want to use for the resulting image.

-

Extract All

If selected, all parts of the corresponding image are extracted.

-

Extract Single

If selected, you can select a single image to be extracted.

-

Extract Range

If selected, you can select a certain range of images to be extracted.

-

Extract Multiple

If selected, you can select several continuous ranges and individual sections.

Enter one or more sections that you want to select in the input field. To do this, enter the first section, followed by a minus sign, and then the last section. If you want to define an interval, after the last section enter a colon and then the interval. The entry "2-10:2" means that every second section is selected from section 2 to section 10.

Enter a comma after the first section if you want to define another section. You can also select individual sections separated by commas. By entering "2-10:2,14-18,20,23", you select every second section from section 2 to section 10, followed by sections 14 to 18, as well as sections 20 and 23.

-

Get current position

Adopts the position from the current display in the image area.

-

Interval

Activated: Interval mode is active. The Interval spin box/input field appears.
Enter the desired interval here. E.g. if you enter the value 2 only every 2nd value from the range is considered.

Region

Here you can select if you want to use the entire image or just a region (ROI) of the input image. Note that in combination with image dimensions, the rectangle is only drawn into one and only this is considered for export. For example, in an image with multiple scenes, the image export only considers the ROI that is always drawn into the currently displayed scene defined by the Dimensions view options tab.

-

Full

If selected this option, the full image is used for the new image.

-

Rectangle Region (ROI)

If selected this option, you can draw in a rectangle region of interest which is used for creating a new image.

If a rectangle region is drawn in, you can see and change its coordinates by editing the input fields for X, Y, W, and/or H.

Tiles

Not available for the OME TIFF Export.

-

Export Selected Tiles

Exports the tiles according to the selected original image.

-

Crop to Selection and Generate New Tiles

Creates new tiles according to your entries in the fields Columns/Rows and Overlap.

-

Columns/Rows

Only available if Crop to Selection and Generate New Tiles is activated.
Defines, with how many rows and columns the re-tiling is performed.

-

Overlap

Only available if Crop to Selection and Generate New Tiles is activated.
Defines the overlap of the re-tiled tiles in %.

Export to Section

Parameter

Description

Export to

The path of the export folder is displayed automatically in the display field.
To change the file path, click on the Dark rounded square button with three white horizontal dots button to the right of the display field.

Parameter

Description

Prefix

Here you can edit the prefix specified or enter a new name. The name of the original image is specified by default.

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