This module enables you to create an automated workflow to acquire overview images, detect relevant objects with an image analysis and re-image these positions, using another experiment. Additionally, you can set up the Guided Acquisition to automatically repeat the detailed scan or the entire workflow to observe changes over time, such as the growth of organoids or embryos.

Guided Acquisition Workflow:

1. Scan or inspect a large area (or over a long period of time).
2. Perform an analysis to detect interesting objects.
3. Acquire detailed images for every detected object.

A possible application is to detect rare events, for example to find transfected cells. For example, the sample contains many cells that are stained with a blue dye, but only a few are additionally expressing GFP. Guided Acquisition allows you to find these cells and run another, for example high-magnification, experiment on these positions.

After performing a (low-magnification) overview scan, the image analysis detects all cells and determines which of them are expressing GFP, i.e. show a certain intensity in the GFP channel. Then the microscope revisits all GFP-expressing cells and performs a second acquisition there, for example with higher magnification, a z-stack, etc. The analysis results are automatically saved to a folder.