The most important microscope parameters for PSF generation that are not channel-specific are displayed in this section.

If you enter incorrect values, this can lead to incorrect calculations. If the values here are obviously wrong or values are missing, check the configuration of your microscope system.

Parameter

Description

NA Objective

Displays the numerical aperture of the objective.

Immersion

Displays the refractive index of the immersion medium. Note that this can never be smaller than the numerical aperture of the objective. You can make a selection from typical immersion media in the dropdown list next to the input field.

Scale Lateral

Displays the geometric scaling in the X/Y direction.

Scale Axial

Displays the geometric scaling in the Z direction.