1. To perform the experiment, you need the Physiology (Dynamics) module.
2. You have a Sutter DG4/5 with appropriate excitation filters for Fura-2 and a Fura-2 filter set in the microscope's reflector wheel.
3. You are on the Acquisition tab.

1. Create a new experiment in the Experiment Manager, e.g. "Physiology Fura-2".
2. Add the channel Fura-2 using Smart Setup.
3. Activate the Time Series checkbox in the acquisition dimensions.
4. Open the Channels tool.
5. Select the Fura-2 channel from the list.
6. Click on Options and select Duplicate.
7. Select the first Fura-2 channel from the list.
8. Click on the Options and select Rename.
9. You can now rename the channel, e.g. Fura-2 340 nm.
9. Repeat steps 7 and 8 to rename the second channel, e.g. Fura-2 380 nm.
10. Select the Fura-2 380 nm channel.
11. Select another LUT from the dropdown list, e.g. red.
12. Select the entry 21 HE Ex. FURA 380 from the Excitation dropdown list.
13. The excitation filter is used for this channel.
13. Adjust the exposure time and focus for both channels.

1. You have created the channels for your experiment.