Take care that the Auto checkbox on the Dimensions tab is deactivated.

  1. You have started the Acquisition Wizard via the CAT tool.
  2. You are in step 1/7 Overview Imaging.
  1. Check if Image Acquisition mode is selected. This is the default setting when entering the wizard.
  2. From the Experiment dropdown list select the experiment that you have prepared in advance.
  3. From the Objective list select an objective with a low magnification, e.g. 5x.
  4. Select the Channel and the Light Source you want to use for acquiring the overview image. For the overview image we recommend selecting Phase contrast as channel mode.
  5. Move the stage to the upper left corner of your sample.
  6. Click on Set start position to define the starting position of the overview image.
  7. Move the stage to the bottom right corner of your sample.
  8. Click on Set end position to define the end position of the overview image.
  9. Click on Acquire Overview Image.
  10. The overview image will be acquired. Then you should see the complete sample showing all ribbons you want to image.
  11. Click on Apply Stitching to remove the offset between the single tile images.
  1. You have successfully acquired the overview image. You can now continue with the next step by clicking on Next.