1. You are on the Acquisition tab in the Tiles tool.
  1. Start the Live mode to use the stage to locate a point that you want to be at the center of your tile region.
  2. Bring the specimen into focus using the focus drive.
  3. Open the Tile Regions section.
  4. The Tiles mode is activated by default.
  5. Enter the number of tiles you want in the X and Y input fields, e.g. X = 3, Y = 3 equals a tiles region containing 9 tiles.
    Alternatively, you can enter the size of the tile region that you want to add. To do this, activate the Size mode.
  6. Click .
  7. The tile region is added to your experiment. The current stage position and focus determines the center and the z-position of the tile region.
  8. To add further tile regions, move the stage to another position on the sample and repeat the previous steps.
  9. The added tile regions (TR1, TR2, etc.) are displayed in the tile regions list.
  10. If you scroll to the right in the table, you can read the Size of the tile regions.
  11. Save the experiment. To do this, in the Experiment Manager click and select Save As. Enter a name for the experiment in the input field (e.g. Simple Tile Experiment).
  12. Click Start Experiment.
  13. The Tile Region experiment is acquired.
  14. The individual tile regions are displayed in the acquired file as scenes and can be selected using the Scene slider on the Dimensions tab. If you deactivate the Scene checkbox, all tile regions are displayed as an overview.
  1. You have successfully set up and acquired a simple Tile Region experiment.

Z values

To ensure that the individual z values of the tile regions are taken into account, ZEN automatically selects the most appropriate focus strategy when the checkbox Tiles is activated. For the experiment described here no further modification needs to be made. If you want to acquire all tile regions at the same z-position, then you have to select None from the dropdown list in the Focus Strategy tool. The individual z-positions are then ignored and the current z-position at the time the experiment is started is used for all tile regions.

Shortcut

You can also add a predefined tile region at the current stage position by pressing the F9 button on your keyboard. The size of this region is the last defined number of tiles in x and y, or a square of 3x3 tiles if you have never defined a region before.