1. If you are using Direct Processing on different computers, you have connected acquisition and processing computer, see Connecting Acquisition Computer and Processing Computer.
  2. To ensure that the processing computer reads incoming files and starts the processing, on the Applications tab, in the Direct Processing tool, you have clicked Start Receiving. This is usually active by default.
  3. On the Acquisition tab, you have set up your experiment for image acquisition.
  4. On the Acquisition tab, Direct Processing is activated. This activates the Auto Save tool as well.
  5. Depending on your settings, you have defined the folder where the acquired images are stored in the Direct Processing or the Auto Save tool. Use a folder to which the processing computer has access. For information about sharing a folder, see Sharing a Folder for Direct Processing.
  1. On the Acquisition tab, open the Direct Processing tool.
  2. If no Direct Processing settings were made before for the current experiment, a particular processing function is already preselected depending on your microscope, channel settings and licenses.
  3. From the Processing Function dropdown list, select the processing function you want to use.
  4. The parameters of the function are displayed and the name of the function is displayed in the pipeline container.
  5. Set all the parameters of the function for your experiment. For detailed information about the parameters refer to the descriptions of the individual image processing function.
  6. To adjust the parameters individually for each channel of a multi-channel image, activate Adjust per Channel.
  7. The tool displays all channels as a list and the currently selected channel is highlighted. For each channel you can use a dropdown to select whether to process it or to skip it (the channel is not processed and not part of the output image).
  8. To add another function or several other ones, click Add Function.
  9. A new container is added in the pipeline.
  10. Select the next pipeline container, select a processing function from the dropdown, and set the parameters for each function.
  11. You have added and set up a sequence of processing functions.
  12. Click Start Experiment to run the experiment. Note: You can pause the processing. If you stop the experiment, requests that have been sent earlier by the acquisition computer are not processed. However, already processed images will be retained.
  13. The images are stored in the folder you have defined in the Auto Save or Direct Processing tool. When you abort the acquisition, the remote processing will not take place. In case you have set up several processing functions, only the acquired image and the final output image are stored.
  14. The processing computer reads incoming files and starts the processing. The path to the selected folder, the currently processed image as well as the images to be processed are displayed in the Direct Processing tool. The processed image is saved to the same folder specified in the Direct Processing tool. If the image name already exists in this folder, the new file is saved under a new name <oldName>-02.czi.
  15. To cancel the processing on the processing computer, on the Applications tab, in the Direct Processing tool, click Cancel Processing.
  1. Once processing is finished, you are notified on the acquisition PC and can open and view the acquired image as well as the processed image. This should be done on the processing computer, so that you can immediately start a new experiment on the acquisition computer. However, you can also automatically open the processed image on the acquisition PC with the respective setting in the Direct Processing tool on the Acquisition tab.
  2. Information about Direct Processing (e.g. the duration) is available on the Info view tab of the processed image.