With this method you can access the super-resolution data in images acquired with Airyscan.

Note that starting with ZEN 2.5 blue edition, the black border of the processed image is automatically removed. Hence the resulting image will be smaller by 24 pixels in X and Y dimension.

Parameters

Parameter

Description

3D Processing

This option is only available for images with 5 or more z-positions.

If activated, this option improves the resolution in axial and lateral direction. The data set needs to have at least 5 z-sections acquired with an optimal step size. 3D Processing is slower than 2D Processing. For 3D Processing, the whole z-stack (single channel and time point) needs to fit into the physical memory.

2D SR Processing

This function is available for 2D images only. It enhances the 2D resolution.

Note this only results in increased superresolution when images are acquired with optimal settings and sufficient signal.

Auto Filter

If activated, a suitable Super Resolution parameter for the Airyscan processing is automatically determined for the selected data set.

To manually adjust the Super Resolution parameter, deactivate the checkbox. Then determine suitable values by using the corresponding function in the Airyscan viewer in the Airyscan view. Note that the preview is only suitable for 2D Airyscan processing. A preview for 3D Airyscan processing is not available. For adjusting 3D processing parameters, you should first process your data set once using the Auto Filter and then check the value that was actually applied by the Airyscan processing function. This value is stored in the metadata of the processed image and can be accessed using the Info view.

Note: High strength might look attractive at some images, Z planes or color channels, but other filtering artefacts might occur which appear like small rings in the image. Also, the results will become very sharp, but grainy. So carefully check your image data in order to avoid such artefacts.

Adjust per Channel

Only visible, when the Auto Filter is deactivated.

Only available for images with two or more Airyscan channels.

If activated, you can manually set channel-specific Airyscan processing parameters.

Strength

Use this option for an increased (high) or decreased (low) strength of the automatically assigned filter value. This is especially useful for 3D processing, as the 2D preview of the processing filter value in the Airyscan viewer does not allow to conclude the result after a 3D data processing.

The increment of this parameter is ± 0.4 compared to the standard auto Airyscan processing. This setting is not available when manual processing strength is selected.