In the Mean ROI view you can draw ROIs and measure their intensity profile after acquiring time series experiments. The intensity profiles are displayed as charts and can be exported to data tables.

  1. The Physiology (Dynamics) module activates additional features to those of MeanROI for the offline analysis of physiology experiments, e.g. Ratio functions and ROI tracing.
  2. In this view the Image area is always to the left, charting area always to the right. Depending on which Region layout you have selected in Layout tab, the MeanROI view can have a different appearance.
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1

Image area
Here you see the images for each channel of the time series and the ratio image (if the ratio calculation is enabled). The display of images can be adapted in the Layout tab.

2

Charting Area
Here you see the charts for the values of all channels selected in the Layout tab as well as for the ratio calculation (if it is enabled on the Ratio tab). If a ROI is selected in the image area on the left, the corresponding plot line is highlighted (the plot line is thicker) in the charts. The lines in the merged channel image can either have the color of the ROI, or the color of the channel (option available on the Charts tab).

Playhead (blue line)
Indicates the current frame of the time series visible in the image panel(s). The position is synchronized with the displayed image frame number and vice versa and it can be moved via drag & drop. The current time point of the visible frame is displayed to the right of the playhead line in the same time unit as the x-axis of the chart.

3

Table
Displays the values for all channels and regions at the different time points, as well as the temperature, focus (if present in the image metadata) and information about markers. If you deactivate a channel in the Visible Charts section of the Layout tab, the corresponding columns are hidden in the table.
This table is synchronized with the image view and the charts. If you select a field in the table, the corresponding ROI is selected in the image view and the charts (playheads) are updated accordingly.

4

Time Line View
This view is activated in the Layout tab. The chart supports similar functions as the other charts in MeanROI. Here you can limit the time range with the zoom functionality. The actions in this chart are synchronized with the others in the MeanROI view.

5

View Options
Here you have your standard view options as well as specific options for MeanROI, for example for the Layout or the calculation of the Ratio.

Hover over the plot with the mouse (crosshair). A tool tip appears with details of the intensity value at this position, ROI ID #, Channel, and time point (in currently set time unit of x-axis). Note these values (intensity and time) are interpolated. You can visualize the time points alone a plot by activation of the Show Tick marks function on the Chart tab.