Xia and Liu, Biophys J 81, 2395 (2001)

NFRET = Fcorr/(Dcorr*Acorr)1/2

Displays an N-FRET image with intensities converted from the FRET index calculated for each pixel using the Xia method. This method is like the Gordon method with the difference that the square root of donor and acceptor concentration is used for concentration compensation. The resulting image is properly corrected for variances in the fluorochrome concentration.