This function allows you to import SmartFIB stacks of Crossbeam microscopes into ZEN.

Parameter

Description

Select Files

Opens a file browser to select the images for import.

Z-Spacing

Sets the slice distance in z. Per default, Auto is activated, and the distance is calculated automatically with information from the image metadata of the first and last selected image.
Deactivate Auto to enter a value manually.

XY-Scaling

Sets the scaling in x and y. Per default, Auto is activated, and the scaling is automatically taken from the image metadata of the first selected image.
Deactivate Auto to enter a value manually, e.g. if you import images without scaling information in the metadata.

Sample Angle

Sets the angle of the sample. Per default, Auto is activated, and the sample angle is automatically calculated from the image metadata or set to the default of 54 degree and the image is rendered with a 90 degree tilt (if no information is available in the metadata). In this case the SmartFIB stack will be displayed perpendicular to the images acquired on a light microscope.
Deactivate Auto to enter a value manually, e.g. if you know the original sample angle as set in the SmartFIB software and import images without using the information in the metadata.

Read XY Offsets

Activated: Reads the xy offset of the individual slices from the metadata. Note that this can lead to a slanted z-stack depending on the sample orientation during imaging and the metadata.