Import SmartFIB TIFFs
This function allows you to import SmartFIB stacks of Crossbeam microscopes into ZEN.
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Parameter |
Description |
|---|---|
|
Select Files |
Opens a file browser to select the images for import. |
|
Z-Spacing |
Sets the slice distance in z. Per default, Auto is activated, and the distance is calculated automatically with information from the image metadata of the first and last selected image. |
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XY-Scaling |
Sets the scaling in x and y. Per default, Auto is activated, and the scaling is automatically taken from the image metadata of the first selected image. |
|
Sample Angle |
Sets the angle of the sample. Per default, Auto is activated, and the sample angle is automatically calculated from the image metadata or set to the default of 54 degree and the image is rendered with a 90 degree tilt (if no information is available in the metadata). In this case the SmartFIB stack will be displayed perpendicular to the images acquired on a light microscope. |
|
Read XY Offsets |
Activated: Reads the xy offset of the individual slices from the metadata. Note that this can lead to a slanted z-stack depending on the sample orientation during imaging and the metadata. |