This function stitches heterogeneous CZI images together to create a new, single homogeneous time series containing all dimensions and time points in their proper order. This differs from the Time Concatenate function, which simply pastes one time lapse series to the end of another without regard for the proper time order or channel content.

Missing images can either be filled with copies of the previous valid image in the series or filled with black images.

When combining Z-stack time series with non-Z-stack time series a choice can be made between either using only the center plane of the Z-stack or creating an extended focus projection of the Z-stack before stitching the images together.

Parameter

Parameter

Description

Fill Missing with

-

Previous

Fills a missing dimension index with a copy of the last existing image from that index.

-

Black

Fills a missing dimension index with a black image.

Z-Stacks

-

Collapse (EDF)

Reduces a z-stack with an extended focus function to a single plane image which is then added to the output.

-

Collapse (Center Plane)

Only uses the center plane from a z-stack for the output image.

-

Expand

Copies the z-stack to the output unchanged, fills the missing indices according to the setting in the Fill Missing with dropdown list.