In this topic we will show you how to crop a region of interest (ROI) of an image.

1. You have acquired or opened a multichannel image.

1. On the Processing tab, open the Method tool and under Utilities select the entry Create Image Subset.
2. In the Image Parameters section, open the Input tool and select the image.
3. In the Method Parameters section, open the Parameters tool.
4. Open the Z-position dimension setting and select Extract Range of the drop-down list. Set the start position and the end position using the slider or the input field.
5. Open the Region setting and select Rectangular Region of the drop-down list.
6. Click in the image on the start position of the ROI and draw a rectangular region.
   
7. You have marked the ROI which should be cropped.
7. Click on the Apply button.

1. The marked ROI with the defined dimensions is extracted of the image. You can now save or process this image.