In step 1 of the wizard, the following options should be activated to follow our recommended workflow:

1. Check if the Automatic movement to next marker checkbox is activated.
2. This will automatically move the stage to the next marker position after you have confirmed the position of the marker and clicked on Next.
2. Check if the Use automatic marker detection checkbox is activated.
3. The software will try to find the correct positions of each marker automatically.
3. If you need to change the marker color, or check if the marker orientation is set correctly, activate the Use settings for marker detection checkbox to access these functions.
4. Click on Next to move to the next wizard step.