This method allows you to apply arithmetic operations to images in the form of a calculator.

You can process a single image or combine two images.

All operations are performed pixel by pixel.

Parameters

Parameter

Description

Channel Input 1

Here you can select whether you want to use an individual channel or all channels of the first input image for the calculation.

Channel Input 2

Here you can select whether you want to use an individual channel or all channels of the second input image for the calculation.

First Images

Activated: For the second input image uses only the first time points of a time lapse image for the calculation. This allows you, for example, to normalize a time lapse image to the intensity values of the first time points.

Enter the number of images that you want to be used for the calculation using the input field.

Formula

Enter the calculation formula here using the keyboard and numeric keypad. Use "S1" as a placeholder for the first input image and "S2" for the second input image.

Input 1

Inserts the placeholder for the first input image into the Formula input field at the current cursor position.

Input 2

Inserts the placeholder for the second input image into the Formula input field at the current cursor position.

Absolute Intensities

Activate this radio button if input image 1 and input image 2 have the same pixel type.

Normalize Intensities 0..1

Activate this radio button if input image 1 and input image 2 have different pixel types. To allow such images to be combined, the intensity values of the two images are normalized to the value range from 0 to 1 before the calculation.

Operators...

Opens a list of all available operators. Here you can select the operator that you want. If you double-click on a list entry, it is inserted into the Formula input field at the current cursor position.

Delete

Deletes the contents of the Formula input field.

Undo

Undoes the last entry in the Formula input field.