1. You have read the Workflow Physiology (Dynamics) Experiments chapter.

1. In the MeanROI setup, select the Online Ratio tab in the view options .
2. Activate the Activate Live Ratio Generation checkbox.
3. In the Method dropdown list select a method for the ratio calculation. If you select the Single wavelength (F/F0) method for the calculation of the online ratio, you need to define a reference image. See Calculating the Online Ratio for Single Wavelength.
4. If you want to use background correction for the calculation of the online ratio, activate the desired entry under Background Correction. Note that activating Ratio fruitions in Online Ratio/ Ratio tab deactivates the background correction settings on MeanROI tab, i.e. the background functions of these tabs are mutually exclusive.
5. To allow you to apply a constant background value (Constant entry), an input field, in which you can enter the desired value, appears in the formula for the ratio calculation.
6. The ROI entry can only be selected once you have defined at least two ROI in your image.
5. Under Calculate complete the formula for calculating the online ratio by selecting the desired entries from the dropdown lists and indicating values in the input fields.
6. Activate the Threshold checkbox, if you want to set a threshold in your experiment. For information on the calculation of the ratio value R in relation to thresholds, see Calculation of the Ratio Value R.
7. You can adjust the settings for the ratio calculation as required and the preview image on the MeanROI setup is adapted accordingly. Press the Snap button if you need to update the images.

1. You have successfully activated/adjusted the calculation of the online ratio.