Select this focus strategy to use the definite focus device to stabilize the focus in the event of temperature fluctuations during your Time Series experiments.

1. To use the Definite Focus focus strategy, you will need the Definite Focus hardware device.
2. You have completed the general preparations for using focus strategies.
3. You are on the Acquisition tab in the Focus Strategy tool.

1. Select Definite Focus as focus strategy from the dropdown list. Note that Z values defined by Tiles Setup are ignored.
2. In the Stabilization Event Repetitions and Frequency section select Standard mode. This mode will use our recommend default settings for stabilization. When selecting the Expert mode, you can adjust all settings according to your needs.
3. Set up a Time Series experiment, see Acquiring Time Series Images.
4. Use the Live mode to set the focus position using the focus drive.
5. To start the experiment, click on Start Experiment.
6. Definite Focus is initialized at the start of the experiment at the current focus position. The focus is then stabilized in accordance with your settings during the time series experiment. You will be reminded to set the focus accordingly prior to the experiment starting. You can do this by navigating to a suitable location (position or Tile region) and starting live or continuous. You can then continue with the experiment or cancel it.

1. You have successfully used the Definite Focus to stabilize the focus during a Time Series experiment.