1. You have read the Workflow Physiology (Dynamics) Experiments chapter and set up an experiment in MeanROI Setup.
  2. You are on the Acquisition tab.
  1. Start your Physiology experiment by clicking on the Start Experiment button.
  2. The time series experiment is started. The MeanROI View (online) opens and displays the current images and the intensity curves for each ROI measured online. The intensity curves are displayed in the Time Line View and in the diagrams. Note that the MeanROI view will display at the third time point. This is noticeable when the interval time is longer. Thus this display delay should fall into the typically base line of this type of experiments, i.e. prior to the first stimulus of the sample.
  3. You can pause the experiment at any time by clicking on the Pause Experiment button and continue it again by clicking on the Continue Experiment button.
  4. The Focus can be adjusted during the experiment. To prevent images that are not sharp being acquired, pause your experiment and use the Live acquisition button to adjust the focus. Then continue the experiment. Note that using the Live view only works with experiments run in interactive mode. In triggered acquisition scenarios this is not possible.
  5. Adjust the display of the intensity values during the experiment by changing the settings on the Layout or Charts tab. The unit of the X-axis cannot be changed during the experiment.
  6. You can move and change ROIs during acquisition. The changes are adopted for all time points, see Drawing in and adjusting ROIs. Note that ROI tracing functions (these allow objects to be followed in XY) are only available after an acquisition.
  7. Activate Switches in the Time Series tool during the experiment to perform the corresponding actions.
  8. Various events, such as the activation of switches or the pausing of the experiment, are labeled in the Time Line view by markers.
  9. On the Dimensions tab deactivate the Follow Acquisition checkbox to analyze the data acquired up to that point. To do this, select the corresponding time points using the Time slider, the diagram sliders or the Time Line view slider in the MeanROI view.
  10. Change the size of the area marked in blue in the Time Line View to adjust the section displayed in the charts (time axis).
  1. You have successfully started the experiment, analyzed it online and influenced it.