If you add positions or tile regions, the current Z-value is automatically adopted for the tile region or position.

To ensure that the individual z values of the tile regions and/or positions are taken into account, ZEN automatically selects the most appropriate focus strategy when the checkbox Tiles is activated. For the experiment described here no further modification needs to be made. If you want to acquire all tile regions at the same z-position, then you must select None from the dropdown list in the Focus Strategy tool. The individual z-positions are then ignored and the current z-position at the time the experiment is started is used for all tile regions. In the case that you do not have the Tiles & Positions module, ZEN will still select the appropriate focus strategy for you.