With this function you can align (or overlay) two images that are displaced in relation to each other. It is also possible to make the individual planes of a z-stack image congruent, in the event that they are not lying exactly on top of one another.

You can define 3 related points (Input Pixel) in both the input image and in the reference image (Reference Pixel) that is displaced in relation to it. Therefore, click interactively on conspicuous points, which are present in both images. If you click Apply, the function calculates the Output image, in which the new fitting points have the same coordinates as in the Input image.

Parameters

Parameter

Description

Input Pixel 1 - 3

If you click on the corresponding buttons, you can define the 3 input pixel points.

The selected point is shown in the graphics plane. This serves as an aid to orientation when you are clicking on the reference points.

Reference Pixel 1 - 3

If you click on the corresponding buttons, you can define the 3 reference pixel points.

Interpolation

Here you can specify how the rotation influences the neighboring pixels.

-

Linear

The rotated pixel is given the gray value calculated from the linear combination of the gray values of the pixel closest to it and this pixel’s nearest neighbor.

-

Cubic

The rotated pixel is given the gray value resulting from a polynomial function of the pixel that is closest to it.