After selecting Image Acquisition in the Layer Thickness Measurement tool, the Layer Thickness Measurement wizard consist of three steps:

Step 1/3: Setup

In this step you choose an experiment or setup the imaging parameters for a new z-stack acquisition, e.g. track setup, acquisition mode, z-stack settings, see Step 1: Setup.

Step 2/3: Sectioning

In this step (orthogonal sectioning) you define the cross section of the sample, you want to analyze. Here, in addition to the center view (X/Y axis; blue frame), you will also see the section views of the X/Z axes (top, green frame) and Y/Z axes (right, red frame), see Step 2: Sectioning.

Step 3/3: Measurement

In this step you draw one more intensity profiles in your cross section. In these profiles you can measure and create tables containing the measurement results. Finally you can save the profile, the z-stack or the result table, see Step 3: Measurement.