These instructions explain how to deconvolve a z-stack image correctly step by step.

In the described example, the best method Constrained Iterative and a theoretical PSF are used.

Preparation

To follow these instructions you will need a z-stack image of your sample. You have opened the software and no images are loaded.

Prerequisites

• You have licensed this function and activated it under Tools > Toolkit Manager.
• You are on the Processing tab.
• You have acquired or opened a fluorescence image on which you wish to perform deconvolution.
• All tools are in Show All mode.

Steps

• Step 1: Load input image
  This section describes how to load an input image in Deconvolution (Configurable).
• Step 2: Set parameters
  This section describes how to set the parameters.
• Step 3: Process image
  This section describes how to process the image and compare it with the input image.
• Step 4: Reuse deconvolution parameters
  This section describes how to reuse the deconvolution parameters from an already processed image.