This step is used for reviewing the single images of a Z-Stack. This is necessary because certain images might not be useful for 3D reconstruction due to problems during the image acquisition or sample preparation issues (wrinkles or ruptures within the section). These regions can be replaced either by the previous image or by the following image. To review the images, the images can be displayed as single 2D images in the 2D view or as images series in the Gallery view.

Parameter

Description

2D View

If selected, you can review the single images of a Z-Stack image by using the 2D view.

You can use the Z-Position slider to navigate through the single images.

To replace an image select the image and click whether on the Replace with next or Replace with previous button.

If you click on the Undo button, the last action performed will be undone.

Gallery View

If selected, you can review the single images by using the Gallery view. The single images of a Z-Stack image are displayed as an image gallery.

If you found an image that does not meet your expectations, simply select the image and replace it by the next or previous image.