In this section you find all settings that are channel specific. This means that they can be configured differently for each channel.

Parameter

Description

Use External PSF

Activated: Uses an external measured PSF. You find an additional input window under Image Parameters > Input where you can select the external PSF file. The software checks if the PSF's microscope parameters match with the input image. Deviations (10nm deviation in wavelength will be accepted) make the software use a theoretical PSF.

Attach to Input

If an external PSF was selected, you can attach the file to the input image. The saved input image then contains the correct measured PSF. Usage of a theoretical PSF is possible as well for such an image. Just deactivate the Use External PSF.

Illumination

Displays the excitation wavelength for the channel dye [in nm] by using the peak value of the emission spectrum. The color field corresponds to the wavelength (as far as possible).

Detection

Displays the peak value of the emission wavelength for the channel dye. The color corresponds to the wavelength (as far as possible).

Sampling Lateral

Depends on the geometric pixel scaling in the X/Y direction and displays the extent of the oversampling according to the Nyquist criterion. The value should be close to 2 or greater in order to achieve good results during DCV. As, in the case of widefield microscopes, this value is generally determined by the objective, the camera adapter used and the camera itself, it can only be influenced by the use of an Optovar. With confocal systems, the zoom can be set to match this criterion.

Sampling Axial

Depends on the geometric pixel scaling in the Z direction and displays the extent of the oversampling according to the Nyquist criterion. The value should be at least 2 or greater in order to achieve good results during DCV. This value is determined by the increment of the focus drive during acquisition of Z-stacks and can therefore be changed easily.

Pinhole

Only available if a confocal microscope has been entered under the microscope parameters.

Displays the size of the confocal pinhole in Airy units (AU).

NA Cond.

Only visible if the microscope is a Conventional Microscope and Transmitted Light has been selected as the illumination type.

Displays the numerical aperture of the condenser with which transmitted-light acquisition was performed.