Type of Sample Carrier/Cover Glasses

We recommend cover glasses coated with Indium tin oxide and fiducials. Indium tin oxide minimizes charging effects in the scanning electron microscope. Cover glasses with fiducials enables additional preparation steps after imaging the sample with the light microscopes and before imaging with a scanning electron microscope.

Deposition of Serial Sections on the Cover Glass

• Sequence of serial sections
  During serial sectioning, make sure that you know the sequence of the ribbons as well as the start and the end point of the ribbons.
• Positioning of serial sections on a cover glass
  It is important to position the serial sections in the center of the cover glass. If the sections are too close to the edge of the cover glass, it might happen that the objective touches the sample carrier during the image acquisition. This might happen particularly for immersion objectives. The consequence will be that the focus map is not calculated in the correct way or images are out of focus.
• More than one ribbon (serial section) on a cover glass
  Take care that the single serial sections are not in close contact to each other, this might confuse the numbering algorithm of the software and creates a wrong numbering.