Features

Analysis Setting for Experiment Feedback

Features you have defined are available from within the Feedback Experiment. Any time you change the image analysis settings, you need to reload the *.czias file in the feedback script editor to activate the changes. The image analysis settings are typically saved in the ProgramData folder on your hard drive, e.g. C:\ProgramData\Carl Zeiss\ZEN\Users\XXXX\Documents\Image Analysis Settings.

In this step you select the measurement features.

Parameter		Description
Interactive		Activated: The features can be changed interactively while the analysis setting is run with Start Interactive Analysis.
Classes		Selects the class for which you want to define measurement features. For a 2D analysis setting, the tree view has two entries for each class for which you can define features. The first entry ("Classes") concerns the collection of the objects belonging to the class. The second entry ("Class") represents all individual objects belonging to the class. The typical features of "Classes" are statistical values of the measurement feature over all single objects of that class. For example, Mean Intensity channel 1 gives you the mean intensity of all single objects. Area gives you the sum of all areas (i.e. the total area) of the individual objects. Fo a 3D analysis, each class has only one entry in the tree view for which you can define features for individual regions of the class and features for all regions of the respective class.
Features of Individual Regions/Features of All Regions		Displays the selected features for the currently selected class and allows you to select additional features. Depending on the class selection, you can select and see the features for individual regions or all regions.
-	Select	Opens the dialog for feature selection.
-	Copy to All	Copies the defined features to all other classes.
-	Feature	Displays the feature name(s) added for the currently selected class.
-	Display	If you activate Display in the feature selection dialog for a feature, the result of the measurement is displayed next to the corresponding object in the analyzed image.
Annotations		Only visible for 2D analysis settings and if in the tree view a "Class"-node (i.e. the node concerning the individual regions) is selected. Displays the selected annotations for the currently selected class and allows you to select features which will be shown as annotations in the analyzed image. An example is the center of each region.
-	Select	Opens the dialog for feature selection.
-	Copy to All	Copies the annotations to all other classes.
Annotation Options		Only visible for 2D analysis settings.
-	Color	Activated: Allows you to select the color for the region annotations.
Custom Feature		Opens the editor to define a custom feature, see Creating Custom Features.
Feature list		Displays a list of all created custom features.

Select Features of Individual Regions Dialog

For a description of individual measurement features, see Measurement Features.

Parameter		Description
Selected Features		Displays all selected features that are calculated for the object during image analysis.
-	Name	Displays the name of the respective feature.
-	Display	Activated: The value of the feature for each object is displayed in the analyzed image.
-	Copy	Only visible for Classes (collection of objects) and if more than one class exists. Selects where the feature is copied to. If the Copy column is empty, the selected feature is not copied to any result table. For a description of the possible copy operations, see Copy Operations for Features.
-	Delete	Deletes the currently selected feature from the list.
-	Move Up	Moves the currently selected feature one position up in the list.
-	Move Down	Moves the currently selected feature one position down in the list.
Available Features		Displays the list with all available features. Double-click on the feature to add the feature to the list of selected features on the left.
Search Features		Here you can enter parts of the name of the feature that you are looking for. The features in which the entered character string occurs are listed. Select a type of feature according to which you want the features to be filtered from the dropdown list.
-	All	All features are listed.
-	Geometric Features	All geometric features are listed.
-	Intensity Features	All features that analyze intensity values are listed.
-	Image Features	All features that contain meta information about the measured image are listed.
-	Position Features	All features that describe the position are listed.
-	Geometric Features Unscaled	All features that describe unscaled geometric features are listed.
-	Position Features Unscaled	All features that describe unscaled positions are listed are listed.
-	Polygon-based Features	All polygon-based features are listed.
-	Statistical Features	All features that can be used for plotting in a heatmap (i.e. that provide statistical values suitable for heatmap plotting) are listed.
Add		Adds the selected feature to the list of selected features on the left.

Select Features of All Regions Dialog

For a description of individual measurement features, see Measurement Features.

Parameter		Description
Selected Features		Displays all selected features that are calculated for the object during image analysis.
-	Name	Displays the name of the respective feature.
-	Display	Activated: The value of the feature for each object is displayed in the analyzed image.
-	Copy	Only visible for Classes (collection of objects) and if more than one class exists. Selects where the feature is copied to. If the Copy column is empty, the selected feature is not copied to any result table. For a description of the possible copy operations, see Copy Operations for Features.
-	Delete	Deletes the currently selected feature from the list.
-	Move Up	Moves the currently selected feature one position up in the list.
-	Move Down	Moves the currently selected feature one position down in the list.
Available Features		Displays the list with all available features. Double-click on the feature to add the feature to the list of selected features on the left.
Search Features		Here you can enter parts of the name of the feature that you are looking for. The features in which the entered character string occurs are listed. Select a type of feature according to which you want the features to be filtered from the dropdown list.
-	All	All features are listed.
-	Geometric Features	All geometric features are listed.
-	Intensity Features	All features that analyze intensity values are listed.
-	Image Features	All features that contain meta information about the measured image are listed.
-	Position Features	All features that describe the position are listed.
-	Geometric Features Unscaled	All features that describe unscaled geometric features are listed.
-	Position Features Unscaled	All features that describe unscaled position features are listed.
-	Statistical Features	All features that can be used for plotting in a heatmap (i.e. that provide statistical values suitable for heatmap plotting) are listed.
Add		Adds the selected feature to the list of selected features on the left.

Annotations Dialog

Parameter		Description
Selected Features		Displays all selected features that are calculated for the object during image analysis.
-	Name	Displays the name of the respective feature.
-	Delete	Deletes the currently selected feature from the list.
-	Move Up	Moves the currently selected feature one position up in the list.
-	Move Down	Moves the currently selected feature one position down in the list.
Available Features		Displays the list with all available features. Double-click on the feature to add the feature to the list of selected features on the left.
Search Features		Here you can enter parts of the name of the feature that you are looking for. The features in which the entered character string occurs are listed. Select a type of feature according to which you want the features to be filtered from the dropdown list.
-	All	All features are listed.
-	Geometric Features	All geometric features are listed.

Custom Feature Editor

Parameter		Description
Custom Features		Displays a list of the created custom features.
–	Name	Displays the name of the created custom feature.
–	Add	Adds a new custom feature that can be defined with the options on the right side.
–	Delete	Deletes the currently selected feature.
Define Custom Feature
–	Name	Defines the name of the feature.
–	Unit	Specifies the unit of the feature as free text input. This input is optional.
Define Operands		Displays and defines the operands used for the calculation of the custom feature.
–	Operand	Displays the name of the operand.
–	Class	Selects the class that is used for the definition of the operand. For a single region class, also the classes of the children can be selected.
–	Feature	Displays all available predefined measurement features for the selected class and selects which measurement feature should be used for the definition of the current operand. If you activate the checkbox behind the selection dropdown, the selected feature is also set visible in the result table.
–	Expression	Displays the expression of the Operand(s) defined by the Class and Feature selection.
–	Add	Adds a new operand to the list.
–	Delete	Deletes the currently selected operand.
Define Custom Expression		Defines the mathematical calculation of the feature, using the Operands and mathematical operators, e.g. *100(a/b+Math.Pow(c,2))**.
–	+	Adds the mathematical operator for summation to the calculation.
–	-	Adds the mathematical operator for subtraction to the calculation.
–	*	Adds the mathematical operator for multiplication to the calculation.
–	/	Adds the mathematical operator for division to the calculation.
–	Pow	Adds the mathematical operator to calculate the power of a certain base value. Note that this operator requires two input values (the base and power value) separated by a comma, e.g. Math.Pow (2,3), which corresponds to 2³.
–	Sqrt	Adds the mathematical operator to calculate the square root of a value.
–	Abs	Adds the mathematical operator to return the absolute number, i.e. non negative values.
Verify Expression		Checks the syntax of the Expression.
OK		Adds the defined custom features and closes the dialog.
Cancel		Closes the dialog without saving the changes.