Differences on Workflow Tab

For workflows, the Features Setup step and tool offer the controls of the wizard step Features. However, the Interactive checkbox is not available, as the workflow defines how the step is executed, see ZEN Workflows.

Analysis Setting for Experiment Feedback

Features you have defined are available from within the Feedback Experiment. Any time you change the image analysis settings, you need to reload the *.czias file in the feedback script editor to activate the changes. The image analysis settings are typically saved in the ProgramData folder on your hard drive, e.g. C:\ProgramData\Carl Zeiss\ZEN\Users\XXXX\Documents\Image Analysis Settings.

In this step you select the measurement features.

Parameter

Description

Interactive

Activated: The features can be changed interactively while the analysis setting is run with Start Interactive Analysis.

Classes

Selects the class for which you want to define measurement features.

For a 2D analysis setting, the tree view has two entries for each class for which you can define features. The first entry ("Classes") concerns the collection of the objects belonging to the class. The second entry ("Class") represents all individual objects belonging to the class. The typical features of "Classes" are statistical values of the measurement feature over all single objects of that class. For example, Mean Intensity channel 1 gives you the mean intensity of all single objects. Area gives you the sum of all areas (i.e. the total area) of the individual objects.

Fo a 3D analysis, each class has only one entry in the tree view for which you can define features for individual regions of the class and features for all regions of the respective class.

Features of Individual Regions/Features of All Regions

Displays the selected features for the currently selected class and allows you to select additional features. Depending on the class selection, you can select and see the features for individual regions or all regions.

-

Select

Opens the dialog for feature selection.

-

Copy to All

Copies the defined features to all other classes.

-

Feature

Displays the feature name(s) added for the currently selected class.

-

Display

If you activate Display in the feature selection dialog for a feature, the result of the measurement is displayed next to the corresponding object in the analyzed image.

Annotations

Only visible for 2D analysis settings and if in the tree view a "Class"-node (i.e. the node concerning the individual regions) is selected.
Displays the selected annotations for the currently selected class and allows you to select features which will be shown as annotations in the analyzed image. An example is the center of each region.

-

Select

Opens the dialog for feature selection.

-

Copy to All

Copies the annotations to all other classes.

Annotation Options

Only visible for 2D analysis settings.

-

Color

Activated: Allows you to select the color for the region annotations.

Custom Feature

Opens the editor to define a custom feature, see Creating Custom Features.

Feature list

Displays a list of all created custom features.