The heatmap displayed in the Analysis View is calculated based on statistical features which you add in the Features step of the Image Analysis Wizard.
The statistical measurement is done on the basis of the top class objects of your analysis setting. Consider the following example:

To calculate, for example, the Mean Area of the Classes 1, the area of all Class 2 objects is summed up and then the total area is divided by the count of objects.

This table shows the offered statistical parameters and the calculation description:

Parameter

Description

Area

Calculates the total area of all objects found in a well or chamber.

Area Percentage

Calculates the percentage that the area of all objects has in respect to the whole frame area (area of all objects divided by the whole frame area).

Count

Calculates the count of objects.

Intensity Std

Calculates the intensity standard deviation divided by the number of objects.

  • sn = Number of the scenes in a well or chamber
  • os = Number of objects found in the scene s
  • xi = Intensity standard deviation of the object i

Mean Intensity

Calculates the mean value of the mean of the intensity and divides it by the number all objects in all scenes.

  • sn = Number of the scenes in a well or chamber
  • os = Number of objects found in the scene s
  • xi = Intensity mean value of the object i

Mean Area

Calculates the mean value of the mean of the area and divides it by the number of all objects in all scenes.

  • sn = Number of the scenes in a well or chamber
  • os = Number of objects found in the scene s
  • xi = Mean area of the object i