Correlation View

This view displays your experiment and the correlation data of the individual measurement positions. The different areas containing the image, the charts and the table can be resized by dragging the individual area boundaries with the pressed left mouse button.

1	Preview Image Here you can see the acquired preview image with the measurement positions marked in the view.
2	Parameter Table This table displays the data for the different measurement positions. Each position is displayed as a row. You can select one or multiple rows, which updates the information displayed in the charts. For details, see Correlation Parameter Table.
3	Charts Here you have the result charts visualizing the measurement data for the selected spots. You can zoom into the displayed curves with the mouse wheel or by drawing a zoom rectangle with pressed left mouse button. A right click in the chart area resets the chart (zoom) to the default view.
4	View Options Here you have the area for your standard view options as well as the specific ones provided on the Correlation Tools tab, see Correlation Tools Tab.

Correlation Parameter Table

This table displays the data for the different measurement positions. Each position is displayed as a separate row. If a value could not be calculated for a specific position, the corresponding entry in the table is empty. A click on an entry highlights and selects the entire row. You can also select multiple rows when pressing the Ctrl button while selecting the entries. The charts are then updated accordingly. The units for individual parameters are displayed as tooltips. A right click in the table opens a specific context menu, see Parameter Table Context Menu.

Parameter	Description
Ø	Activated: Uses this position for the calculation of an average for individual values. The average is displayed on the bottom of the table.
Visibility	Toggles the visibility of the respective spot.
ID	Displays the ID for the individual spot.
Color	Sets the color for the respective measurement point by clicking on the field and selecting a color form the dialog.
Name	Displays and sets the name for the individual position.
Count Rate	Displays the count rate in kHz.
CPM	Displays the CPM in kHz. The color of the value indicates the data quality, see Data Quality Criteria (CPM).
Data Quality	Displays an indication for the fit quality.
Number of Molecules	Displays the number of molecules found at the measurement position. It is calculated by 1/correlation amplitude, where the correlation amplitude is defined as value of the correlation curve at the smallest lag time.
Concentration	Only visible after using a 3D fit model. Displays the concentration.
Surface Concentration	Only visible after using a 2D fit model. Displays the value for the surface concentration in nmol/cm².
Triplet State Fraction	Displays the triplet state fraction. One physical property of the utilized fluorophore. Dependent on intensity and if applicable oxygen. A triplet state of e.g. 16% would mean that 16% of all fluorophores are in dark state.
Chi²	Displays the Chi² value, see also Fit Calculation. Chi² is the deviation between the measured FCS curve and the fit curve. A value of 0 would mean a 100% accordance.
Diffusion Time	Displays the diffusion time.
Diffusion Coefficient	Displays the diffusion coefficient.
α	Only visible if you have used an anomalous fit model. Displays the anomalous parameter: 1 corresponds to free diffusion, lower values indicate constrained diffusion.
Fit Model	Displays which fit model was used.
Date	Displays the date and time of the experiment.

Correlation Tools Tab

Parameter		Description
Reference Image		Only visible if you have created a second reference image after the spot experiment. Selects which reference image is displayed int the view.
–	Pre-Experiment	Displays the reference image acquired before the experiment.
–	Post-Experiment	Displays the reference image acquired after the experiment.
Display Chart		Selects which chart is displayed above the correlation chart.
–	Count Rate	Displays the count rate chart.
–	Residuals	Displays the residuals chart. Note that the residuals chart only contains information after a fit was executed for the respective spot.
Filter
–	Detrending	Activated: Applies a detrending filter to remove the trend (e.g. due to the photo bleaching) from the experiment data. Detrending is calculated by a low-pass filter with the filter time constant provided with the input field.
–	Dust	Activated: Applies a dust filter to remove high intensity peaks (caused e.g. by aggregated objects) from the experiment data and sets the parameter with the input field. Dust is considered, if the average intensity multiplied with the parameter value is higher than the mean signal intensity.
Fit Model		Selects the fit model for the correlation curve.
–	1 Component 2D	Selects the one component 2D model.
–	2 Component 2D	Selects the two component 2D model.
–	1 Component 3D	Selects the one component 3D model.
–	2 Component 3D	Selects the two component 3D model.
–	1 Component Anomalous 2D	Selects the one component anomalous 2D model.
–	1 Component Anomalous 3D	Selects the one component anomalous 3D model.
–	Fit	Applies the fit model to the currently selected position/curve.
–	Fit All	Applies the fit model to all positions/curves.
Amplitude Normalization
–	Activate	Normalizes the amplitude to 1.
–	Deactivate	Applies no normalization.
ω_r Calibration		Selects which ω_r should be used for the fit of the curves.
–	Default	Uses the default setting for the ω_r calibration.
–	Custom	Opens a browser to select a file with a user generated ω_r calibration. This file can be created with the ω_r tab.

ωr Calibration Tab

This tab enables you to create your own custom ω_r calculation that you can use for fitting the correlation curves.

Parameter	Description
Dye	Selects the dye used for the ω_r calibration.
Diffusion Coefficient	Displays and sets the diffusion coefficient for the dye.
Calibrate	Calibrates the ω_r value and opens a file browser to save it as a calibration file.

Correlation View

Correlation Parameter Table

See also

Correlation Tools Tab

See also

ωr Calibration Tab

See also

ON THIS PAGE