1. You have selected an objective with a relatively low magnification in your experiment settings.
  2. You are in Advanced Setup.
  1. In the left toolbar, click on Preview Scan.
  2. The Preview Scan Toolbar is displayed as the top toolbar.
  3. In the top toolbar, deactivate Use Existing Experiment Settings and select/ unselect the channels that you want to use for the preview scan.
  4. If necessary, use the live mode to adjust the focus area and exposure following a change of objective or channel.
  5. To obtain a better overview, slightly zoom out of the Advanced Setup view.
  6. Start the Live mode to use the stage to locate approximately the center of the region for which you want to generate a preview scan.
  7. In the left toolbar in the Tiles section, click on the Setup by contour button.
  8. The Contour Toolbar is displayed as the top toolbar.
  9. In the top toolbar, select the Rectangular Contour tool.
  10. In the Stage View, use the tool to draw a rectangle that approximately encloses the region for which you want to generate a preview scan.
  11. Alternatively, in the Stage View, use the live image to navigate to the edge of the area that you want to image. We recommend, for example, the bottom left corner. Add the first marker. Now navigate to the upper edge of the object and add a second marker. Finally, if necessary, navigate to the right edge and add a third marker and click on Done. You can optimize the tile region by selecting a circular contour shape for your sample instead.
  12. A tile region is created for the marked region and displayed in the list in the Tile Regions section of the Tiles tool.
  13. With the help of the Live mode, check whether the desired image region is covered by the tile region. To do this, use the stage to locate the corners and edges of the tile region and increase or reduce the yellow selection frame as necessary.
  14. In the left toolbar, click on Preview Scan and in the top toolbar click on Start.
  15. A series of snap images is acquired to generate a preview of the marked region.
  1. You have successfully generated a preview scan.

Before you continue with the actual experiment, carry out the following steps:

  1. In the Tiles Regions section, deactivate the preview tile region (TR 1) by deactivating the checkbox of the corresponding list entry. This prevents the acquisition of the preview tile region during the actual experiment.
  2. In the Microscope Control tool in the Right Tool Area, select the objective you want to use for final acquisition.
  3. Use the Live mode to adjust the focus area and exposure accordingly.
  1. You can now continue setting up the tile experiment.