Step 3: Set Exposure Time
This step enables you to set the exposure time for each channel.
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Parameter |
Description |
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|---|---|---|
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Select Reference Slide |
Selects the reference slide with the two drop-downs. The first selects the tray, the second selects the slide. |
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– |
Onboard Slide |
Loads the respective tray and slide, and executes an overview experiment. |
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Autofocus |
Starts an autofocus search with the focus strategy defined in the reference profile. |
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Auto Exposure |
Starts an automatic exposure time measurement for all channels. |
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Live |
Starts a live acquisition and displays the live image in the center area of the step. |
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Snap |
Acquires a single image (snapshot) and displays it in the center area of the step. |
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Channel Table |
Displays all channels of the profile and their exposure time. |
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– |
Marker |
Displays the channel name. |
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– |
Dye |
Displays the dye name. |
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– |
Exposure Time |
Displays and sets the exposure time for the respective channel. |
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– |
Auto |
Starts an automatic exposure time measurement for the respective channel. |
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Reset |
Resets all exposure times to the values of the reference scan profile. |
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Record Exposure Time |
Saves the current exposure time settings and displays them in the list of the section below. |
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Recorded Exposure Times |
Displays a section with saved exposure times. Each recorded measurement is added as a column. |
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– |
Marker |
Displays the marker and the saved exposure times. |
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– |
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Deletes the currently selected entry of saved exposure times. |
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– |
Use Minimal |
Sets the smallest value of the saved exposure times as the exposure time. |
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– |
Use Average |
Sets the average value of the saved exposure times as the exposure time. |
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Back |
Goes back to the previous wizard step. |
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Finish |
Saves the changes and closes the wizard. |
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Cancel |
Closes the wizard without saving. |
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