This topic will show you how to set-up a measurement program using the Image Analysis Wizard. After this the program will be used to count the number of fluorescence spots in a multichannel image.
In this example we are using a multichannel image with 2 channels (1st channel blue (DAPI), 2nd channel green (GFP)) of fluorescence-stained cell nuclei. First, we detect the blue-stained cell nuclei in the first channel and then the green stained signals in the second channel. Then we measure the number of green fluorescence signals per nucleus.