Depending on your loaded image, you can split certain dimensions, e.g. channels or timepoints, and save them to separate images. First, you select the method, and in the Parameters tools, you specify the dimension. Each of the dimensions is only visible if the corresponding dimension is present in the input image.

Create Image Subset and Split opens the resulting images in the ZEN document area. Therefore, for this image processing function, Split Dimension is limited to a maximum of 20, e.g. timepoints, tiles, or slices. To split images along a dimension with more than 20 elements, use Create Image Subset and Split (Write files), which creates image files in the specified folder.

1. You have loaded an image with more than one channel and not more than 20 timepoints.

1. Select Processing tab > Method tool, and select under Utilities the entry Create Image Subset and Split or Create Image Subset and Split (Write files). When using the first option, you can specify the output in the Output tool.
2. To create a subset from the available dimensions, make your settings in the sections Channels and Time.
3. In the Parameters tool, in the Split Dimension section, select the dimension along which you want to split the image, e.g. Channel or Time.
   In the Channels list, all channels of the loaded image are listed. Unselect channels you want to exclude.
   In the Time section, select the subset of timepoints which you want to include. If you have an image with more than 20 timepoints, and you limit the range to below 20 elements, Time will appear in Split Dimension allowing you to split the data set along this dimension (for Create Image Subset and Split).
   In the Region section, select the region which you want to extract from the image, e.g. Full or Rectangle Region.
   Select Rectangle Region and draw a rectangle in the 2D view to specify the region you want to extract.
   When you use Create Image subset and Split (Write files), you also need to specify the output folder for the images.
4. In the Input tool, specify whether you want to set the input automatically and which image you want to display after processing. Check the according options.
5. In the Output tool, specify how to proceed with the new image. Select if you want to overwrite an existing image with the same name or if you want to create a new output. The output tool is only available, if you selected the Create Image Subset and Split method.
6. Click the Apply button.

1. The new images are created according to your specifications, see Create Image Subset and Split or Create Image Subset and Split (Write files).