Some basic functionality for deconvolution is generally available in the software, but the full Deconvolution functionality requires a license.

Basic functionality

The functionality generally available in ZEN includes:

• The 2D background removal function Deblurring which is based on the nearest neighbor algorithm.
• The Deconvolution (defaults) function, which offers three primary deconvolution methods that are automatically adapted to the type of instrument used to acquire the image.

Licensed Functionality

If you have licensed the functionality and activated it under Tools > Toolkit Manager, the following additional functionality is available:

• The additional and more advanced deconvolution method Constrained Iterative in the Deconvolution (defaults) function.
• GPU acceleration with dedicated CUDA-compatible graphic cards, including support for multi-GPU.
• The Deconvolution (adjustable) function, which offers access to all available function parameters and provides the necessary flexibility for demanding samples and sample conditions.
• The creation of a PSF with the PSF Wizard, a function offering a wizard to guide you through a series of steps to create a PSF from a z-stack image of multiple fluorescent beads. This function is recommended to create experimentally measured PSFs.