This step is an optional step. It allows to image the ribbons with an objective with higher magnification, if necessary. E.g. when you would like to define the regions of interest by means of the sample structure that can only be identified using lenses with higher magnification.

Therefore you can mark the outlines of the ribbon on your sample. If you cannot see the sample structures, due to the overview image was acquired with a too low magnification, you can image your sample again using a higher magnification.

For marking the outlines in the image use the tools from the Ribbon Definition tab (e.g. Rectangle, Circle or Polygon).