Calibrated Unmixing Wizard

With this step you select autofluorescence spectra. You have the following possibilities:

Load an xml file which defines the autofluorescence spectrum, for example a file from a previous run of this wizard.
Load an image and draw graphical elements into the image which indicate autofluorescence spectra and background.

If you load an image, it is displayed in the Center Screen Area and you have the 2D and Split view available. If you load an xml file, the chart of the spectrum is displayed in the Center Screen Area.

Parameter	Description
Selection Field	Displays the selected file. Click to open a file browser and select an xml or czi file.
Show Spectrum	Only visible if you have selected autofluorescence and background with the drop-down below. Activated: Displays a chart with the currently selected autofluorescence spectrum in the Center Screen Area. Deactivated: Displays the selected czi image in the Center Screen Area.
Select Autofluorescence	Only visible if you have loaded a czi file and you have drawn a graphic into the image. Selects the graphical element that marks the autofluorescence with the drop-down list.
Select Background	Only visible if you have loaded a czi file and you have drawn a graphic into the image. Selects the graphical element that marks the background with the drop-down list. If there is no background in the image, select None.
Fixed Background	Only visible if Select Background is set to None and if the necessary information are not present in the file metadata. Sets a fixed value for the background. In case of HDR scans the value should be set to 255, in other cases to 64.
Next	Continues to the next step.
Cancel	Cancels and closes the wizard.

See also

Step 1: Selection of Autofluorescence Spectra

See also

Step 3: Calculated Dye Spectra

See also

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Parameter	Description
Back	Goes back to the previous step.
Finish	Saves the new spectra and closes the wizard.
Cancel	Cancels and closes the wizard.