Ratio Tab - MeanROI Offline Ratio & Cache

Ratio Tab

This view option is almost identical to the Online Ratio tab in the MeanROI Setup, see Online Ratio Tab. In fact when an experiment is finished the exact same values used for the display of the online ratio are transferred to the offline ratio tool of the MeanROI view and are stored with your image for later reference.
For offline ratio assessment the settings can be changed and are applied to the ratio image and the measured values. For large data sets (high resolution or many time points), a smooth playback of the ratio image can be achieved by clicking on the Cache ratio image button. This stores the ratio images temporarily to the computers memory for very fast playback or adjustments of the time slider. The following descriptions describe the differences on the Ratio tab:

Parameter	Description
Ft₀= Average intensity of frame	Only available if the Single wavelength method is selected. Defines the frames of the time series image from which the reference value Ft ₀ should be calculated. The numbers in the input fields refer to the frame number of the current experiment in the MeanROI view. The desired frame numbers can be entered with the buttons or directly by typing a number into the field. Typically, your experiment should include a baseline of 5-10 images before and/ or in between a simulation/ activation.
–	Update	Applies the changes of the frame made with the input fields.
Cache ratio image	Caches all the ratio images of the current time series with the given ratio calculation parameters. This is done to avoid image flickering when moving through the time series with the slider quickly.

Parameter

Description

Ft₀= Average intensity of frame

Only available if the Single wavelength method is selected.

Defines the frames of the time series image from which the reference value Ft ₀ should be calculated.
The numbers in the input fields refer to the frame number of the current experiment in the MeanROI view. The desired frame numbers can be entered with the buttons or directly by typing a number into the field. Typically, your experiment should include a baseline of 5-10 images before and/ or in between a simulation/ activation.

–

Update

Applies the changes of the frame made with the input fields.

Cache ratio image

Caches all the ratio images of the current time series with the given ratio calculation parameters. This is done to avoid image flickering when moving through the time series with the slider quickly.