Only visible if the Show All mode is activated.

Parameter

Description

Phase Ring

If you have acquired a fluorescence image using a phase contrast objective, the phase ring present in the objective is entered here. This setting has significant effects on the theoretical Point Spread Function (PSF).

PSF Generation

Selects the model for calculating the PSF.

–

Scalar Theory

The wave vectors of the light are interpreted as electrical field = intensity and simply added. This method is fast and is sufficient in most cases (default setting).

–

Vectorial Theory

The wave vectors are added geometrically. However, the calculation takes considerably longer.

Z-Stack

This field can only be changed if it was not possible to define this parameter during acquisition, e.g. because the microscope type was unknown. It describes the direction in which the z-stack was acquired. Note that this setting is only relevant, if you are using the spherical aberration correction.

–

Descending

The z-stack descends away from the objective.

–

Ascending

The z-stack ascends towards the objective.