1. You have licensed the Spatial Biology toolkit and activated it in Tools > Toolkit Manager.
  2. You are in the Scan step of the Scan Profile Editor.
  3. You have reference spectra available for your unmixing.
  1. In the Online Processing section, activate Unmixing active.
  2. The parameters for unmixing are displayed, see Unmixing Parameters.
  3. For Ref. Spectra, click .
  4. A dialog for the unmixing database opens.
  5. Select the folder containing your reference spectra and click OK.
  6. The reference spectra are loaded.
  7. To change or select a reference file for a channel, go to the table and click for the channel.
  8. A dialog opens.
  9. Select the reference file for the dye of the current channel and click OK.
  10. You have set the reference for the this channel.
  11. Repeat the previous steps for all channels of your experiment.
  12. The reference spectra for your scan are set.
  13. Continue with the remaining setup of your scan profile, see Adjusting Scan Settings and Working in the Scan Profile Editor.
  1. You have defined an unmixing in your scan profile. Unmixing will be applied with your scan profile.