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ZEISS Microscopy Knowledge Base

Performing SIS file optimization

This guide explains how to perform the SIS file structure optimization (Defragmentation).

All the redundancies and the unused spaces are removed and the SIS file compacted and packed. This task allows to reduce the file overall size.

The defragmentation is strongly suggested after any editing task of the Image-Set, Channels, Planes or Time points.

It is also useful when the stitching, the volume fusion or additional dataset import are done. Check the file status always before transferring it to the permanent storage unit or before sharing it by upload to any transfer services.

Two rectangular color-tile grids side by side, left more scattered, right more compact, with a purple curved arrow between them

Opening the working dataset

  1. Select Open... from the file menu.
    File menu showing Open... option highlighted with shortcut CTRL+O
  2. Select the SIS file from the file browser.
    The dataset is a multi dimensional, discrete, representation of your real sample volume. It can be structured as a Z series of planes (Optical sectioning) of multiple channels (dyes) in a temporal sequence of time points (located in several spatial positions).​
    Usually, the dataset shows a single experimental situation (a complete experiment can be composed by several datasets). The datasets are available as graphic files saved in plenty of file formats (standard formats as well as proprietary formats)​.
    Note: The dataset is visualized according to the current rendering setting parameters. Refer to the arivis Vision4D Help for more details.

Tilted grid with colored blob objects; left panel shows 'Blob Finder 2' and 'Segment Filter'

Checking the file status

  1. Select Defragment File from the Data menu.
    Dropdown Data menu showing Defragment File... option highlighted
  2. If the SIS file doesn’t require any optimization, this message is issued:
    Defragmentation dialog reading: The file space is perfectly utilized. A defragmentation is not necessary. OK button visible
  3. Click OK to exit the function.

Applying the defragment task

  1. If the SIS file needs to be optimized, Vision 4D will ask you to select the Defragmentation method.
    Clean-up File dialog: 40% unused space; Fast and Full Defragmentation options; warning icon; Cancel button
  1. Select one of the following methods:
    1. Fast Defragmentation = Performs a fast, but less efficient, task.
    2. Full Defragmentation = A complete optimization is applied. It takes more time. (Suggested)
  2. Wait until the defragmentation is completed.
    Two colored-block grids: left with scattered colors and empty bottom rows, right with stacked colored rows; purple arrow
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