ZEISS Microscopy Knowledge Base
Help
ZEISS Microscopy Knowledge Base

Performing SIS file optimization

This guide explains how to perform the SIS file structure optimization (Defragmentation).

All the redundancies and the unused spaces are removed and the SIS file compacted and packed. This task allows to reduce the file overall size.

The defragmentation is strongly suggested after any editing task of the Image-Set, Channels, Planes or Time points.

It is also useful when the stitching, the volume fusion or additional dataset import are done. Check the file status always before transferring it to the permanent storage unit or before sharing it by upload to any transfer services.

Two color-block grids, left larger with colored rows and gray bottom, purple curved arrow pointing right

Opening the working dataset

  1. Select Open... from the file menu.
    File menu showing Open... option highlighted with CTRL+O shortcut
  2. Select the SIS file from the file browser.
    The dataset is a multi dimensional, discrete, representation of your real sample volume. It can be structured as a Z series of planes (Optical sectioning) of multiple channels (dyes) in a temporal sequence of time points (located in several spatial positions).​
    Usually, the dataset shows a single experimental situation (a complete experiment can be composed by several datasets). The datasets are available as graphic files saved in plenty of file formats (standard formats as well as proprietary formats)​.
    Note: The dataset is visualized according to the current rendering setting parameters. Refer to the arivis Vision4D Help for more details.

Software GUI with 3D volumetric rendering of green and red segmented objects on a grid, left-side tool panels and color scale

Checking the file status

  1. Select Defragment File from the Data menu.
    Data menu dropdown with 'Defragment File...' highlighted under tabs 'Data Navigation Objects Analysis'
  2. If the SIS file doesn’t require any optimization, this message is issued:
    Defragmentation dialog stating 'The file space is perfectly utilized. A defragmentation is not necessary.' with OK button
  3. Click OK to exit the function.

Applying the defragment task

  1. If the SIS file needs to be optimized, Vision 4D will ask you to select the Defragmentation method.
    Clean-up File dialog shows 'The file contains 40 % unused space.' with Fast and Full Defragmentation options and warning
  1. Select one of the following methods:
    1. Fast Defragmentation = Performs a fast, but less efficient, task.
    2. Full Defragmentation = A complete optimization is applied. It takes more time. (Suggested)
  2. Wait until the defragmentation is completed.
    Two rectangular color-block grids side by side with a purple curved arrow pointing from the left grid to the right grid
Impressum
Carl-Zeiss-Strasse 22
73447 Oberkochen
Germany
Legal