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Importing and moving objects in a 3D volume

When importing objects created on another image set, as in the case of atlas regions, it is sometimes necessary to move them to match the destination image set. This article explains how objects can be imported and moved to a new position.

Background

Atlases can be a very useful tool in contextualising segmentation results by allowing the compartmentalisation of objects to specific regions. Creating these regions is often done on a "standard" image set, but when copied over to a new image set slight changes in the sampling can cause a misalignment of the atlas regions with the destination image set. The Copy/Move Objects... function allows the user to move the objects to the correct location. 

Importing objects

If the objects have been exported as an objects database file (.OBJECTDB), they can be imported simply from the Objects menu or the Im/Export button in the objects table.

Application Objects menu dropdown with 'Import Objects...' option highlighted

Objects panel showing 'No objects found.' and Im/Export menu with 'Import Objects...' highlighted

Once imported the objects will appear in the Objects table and the 2D/4D viewers.

3D viewer with transparent grid cube, white particle cloud above a colored translucent segmented object, axes labeled X Y Z (μm)

Moving objects

To move the objects we start by selecting all the objects that need to be moved. To do this, simply select any object in the Objects table then press Ctrl-A on your keyboard to select all. Then, in the Objects menu we can select the option to Copy/Move Objects...

Open Objects menu with Copy/Move Objects... highlighted in 4D Viewer window

This will open a new window where we can select the parameters for the move. In this case we first need to move all the objects 106 planes back up the stack:

Copy or Move Objects dialog showing Plane set to Relative -106 and message Moving 51 objects

Note that the Move option has been selected at the top and we are currently only moving the objects -106 planes relative to their current position. Selecting an absolute position would move every object to the specified plane. 

2D viewer with colored segmented object overlays and dashed selection boxes on dark background

Finally, if an XY adjustment is also required we can use the Move Objects Tool in the 2D viewer to drag them to the correct location:

Toolbar showing Move Objects Tool icon with tooltip in the 2D viewer

3D viewer showing translucent segmented volume inside a wireframe cube with X, Y, Z (µm) axes

Video

Image Courtesy

Kirsty Craigie, The University of Edinburgh.

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